We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Correlations between Risk Factors for Breast Cancer and Genetic Instability in Cancer Patients-A Clinical Perspective Study.
- Authors
Paz, Márcia Fernanda Correia Jardim; de Alencar, Marcus Vinícius Oliveira Barros; Gomes Junior, Antonio Luiz; da Conceição Machado, Keylla; Islam, Muhammad Torequl; Ali, Eunus S.; Shill, Manik Chandra; Ahmed, Md. Iqbal; Uddin, Shaikh Jamal; da Mata, Ana Maria Oliveira Ferreira; de Carvalho, Ricardo Melo; da Conceição Machado, Kátia; Sobral, André Luiz Pinho; da Silva, Felipe Cavalcanti Carneiro; de Castro e Souza, João Marcelo; Arcanjo, Daniel Dias Rufino; Ferreira, Paulo Michel Pinheiro; Mishra, Siddhartha Kumar; da Silva, Juliana; de Carvalho Melo-Cavalcante, Ana Amélia
- Abstract
Molecular epidemiological studies have identified several risk factors linking to the genes and external factors in the pathogenesis of breast cancer. In this sense, genetic instability caused by DNA damage and DNA repair inefficiencies are important molecular events for the diagnosis and prognosis of therapies. Therefore, the objective of this study was to analyze correlation between sociocultural, occupational, and lifestyle risk factors with levels of genetic instability in non-neoplastic cells of breast cancer patients. Total 150 individuals were included in the study that included 50 breast cancer patients submitted to chemotherapy (QT), 50 breast cancer patients submitted to radiotherapy (RT), and 50 healthy women without any cancer. Cytogenetic biomarkers for apoptosis and DNA damage were evaluated in samples of buccal epithelial and peripheral blood cells through micronuclei and comet assay tests. Elder age patients (61-80 years) had higher levels of apoptosis (karyorrhexis by karyolysis) and DNA damage at the diagnosis (baseline damage) with increased cell damage during QT and especially during RT. We also reported the increased frequencies of cytogenetic biomarkers in patients who were exposed to ionizing radiation as well as for alcoholism and smoking. QT and RT induced high levels of fragmentation (karyorrhexis) and nuclear dissolution (karyolysis) and DNA damage. Correlations were observed between age and karyorrhexis at diagnosis; smoking and karyolysis during RT; and radiation and karyolysis during QT. These correlations indicate that risk factors may also influence the genetic instability in non-neoplastic cells caused to the patients during cancer therapies.
- Subjects
GENETICS of breast cancer; DNA repair; CANCER chemotherapy
- Publication
Frontiers in Genetics, 2018, p1
- ISSN
1664-8021
- Publication type
Article
- DOI
10.3389/fgene.2017.00236