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- Title
Cytotoxic CD4<sup>+</sup> T cells specific for Francisella tularensis.
- Authors
Surcel, H. M.; Tapaninaho, S.; Herva, E.
- Abstract
The specific cell-mediated cytotoxicity of tularaemia-immune human T lymphocytes were studied in vitro. Peripheral blood mononuclear cells (PBMC) of six tularaemia-vaccinated healthy subjects were stimulated with F. tularensis LVS whole cell antigen for 6 days and used as effector cells in a conventional 4-h 51Cr release cytotoxicity assay. The target cells were phagocyting autologous monocytes, which were pulsed with F. tularensis or PPD antigen. The specific lysis of the F. tularensispulsed cells (42.6% ± 11.7) was significantly higher (P < 0.05) than that of the PPD-pulsed ones (22.2% ± 8.3) or unpulsed control cells (15.9% ± 5.2), The cytotoxicity was associated with CD4+ F. tularensis-specific T cell clones (TLC), which killed 36.3% ± 12.3 of the F. tularensis-pulsed targets but only 6.9% ± 6.5 of the unpulsed control targets. Their lysing was inhibited by monoclonal anti- HLA-DR and anti-HLA-DQ antibodies, but not by CD 15 (monocyte/macrophage) antibody. The functional role of CD4+ lymphocytes in tularaemia immunity is discussed.
- Subjects
T cells; MONOCYTES; LEUCOCYTES; IMMUNOLOGY; LYMPHOCYTES; VACCINATION; IMMUNOGLOBULINS
- Publication
Clinical & Experimental Immunology, 1991, Vol 83, Issue 1, p112
- ISSN
0009-9104
- Publication type
Article