We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Malaria specific human T cell clones: crossreactivity with various plasmodia species.
- Authors
Simitsek, Phaedra; Chizzolini, C.; Perrin, L.
- Abstract
Peripheral blood mononuclear cells (PBMC) from donors with or without previous exposure to malaria in vivo were cultivated for 4 to 7 days in the presence of different malaria antigen (M.Ag) preparations: Plasmodium faliciparum (P.f.Ag). P. berghei (P.b.Ag)and P. gallinaceum (P.g.Ag). All preparations induced a proliferative response in PBMC from donors with or without previous exposure to malaria. PBMC from both groups of donors were then primed with each of the three M.Ag and cloned in presence of autologous irradiated PBMC and M.Ag. All 152 clones recovered had theT4* phenotype and required aulologous antigen presenting cells (APC) in addition to M.Ag for proliferation. Species specific and crossreactive T cell clones T cell clones proliferating in the presence of the three M.Ag preparations) were recovered following priming with each of the three M.Ag. Species specific and crossreactive T cell clones were recovered in similar percentages (approximately 50p) using PBMC from donors with or without previous exposure to malaria. These data are discussed in the context of crossreactivity at the T cell level among various plasmodia species and in relation lo epitope recognition of malaria native, synthetic and fusion polypeplides.
- Subjects
MONONUCLEOSIS; MALARIA; PROTOZOAN diseases; T cells; LYMPHOCYTES; MYXOMYCETES
- Publication
Clinical & Experimental Immunology, 1987, Vol 69, Issue 2, p271
- ISSN
0009-9104
- Publication type
Article