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- Title
Genetic Polymorphism of the MxA Gene Promoter and Interferon Responsiveness of Hepatitis C Patients: Revisited by Analyzing Two SNP Sites (-123 and -88) in vivo and in vitro.
- Authors
Hijikata, Minako; Mishiro, Shunji; Miyamoto, Chikara; Furuichi, Yasuhiro; Hashimoto, Michie; Ohta, Yasuhiko
- Abstract
We have previously reported a single nucleotide polymorphism (SNP) at nucleotide (nt) position -88 (G or T) within an interferon-stimulated response element-like sequence in the promoter region of the MxA gene, which correlated with responsiveness of hepatitis C patients to interferon. Upstream of it, we then identified another SNP (C or A at nt -123) and investigated whether this SNP also correlates with interferon responsiveness. The two SNPs showed a high linkage to each other: all the individuals having G at -88 had C at -123, and 73% of those having T at -88 had A at -123. As was expected from this observation, the SNP at -123 also exhibited a correlation with interferon responsiveness (C/C homozygotes were more frequent among nonresponders than among responders: 65% of 107 vs. 40% of 52, p = 0.0028). These in vivo data from patients were further supported by results from in vitro experiments. The MxA promoter sequence with A at -123 and T at -88 showed about 4-fold higher activity of upregulating the downstream reporter gene than that with C at -123 and G at -88, in a luciferase reporter assay. Copyright © 2002 S. Karger AG, Basel
- Publication
Intervirology, 2001, Vol 44, Issue 6, p379
- ISSN
0300-5526
- Publication type
Article
- DOI
10.1159/000050075