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- Title
Cytostatic Effects of Polyethyleneimine Surfaces on the Mesenchymal Stromal Cell Cycle.
- Authors
Alba, Anna; Villaggio, Giusy; Messina, Grazia Maria Lucia; Caruso, Massimo; Federico, Concetta; Cambria, Maria Teresa; Marletta, Giovanni; Sinatra, Fulvia
- Abstract
Polyelectrolytes assembled layer-by-layer (PEMs) are commonly used as functional coatings to build-up biological interfaces, particularly suitable as compatible layers for the interaction with a biological medium, providing suitable conditions to promote or prevent cell seeding while maintaining the phenotype. The proper assessment of the biocompatibility of PEMs and the elucidation of the related mechanisms are therefore of paramount importance. In this study, we report in detail the effect of two different PEM endings, polystyrene sulfonate (PSS) and polyethylenimine (PEI), respectively, on the cell adhesion, growth, and viability of human bone mesenchymal stromal cells (MSCs). The results have shown that PSS-ended substrates appear to be the most suitable to drive the cell adhesion and phenotype maintenance of MSCs, showing good biocompatibility. On the contrary, while the cells seem to adhere more quickly and strongly on the PEI-ended surfaces, the interaction with PEI significantly affects the growth and viability, reducing the cell spreading capability, by sequestering the adhesion molecules already in the very early steps of cell–substrate contact. These results point to the promotion of a cytostatic effect of PEI, rather than the often-claimed cytotoxicity.
- Subjects
STROMAL cells; CELL cycle; BIOLOGICAL interfaces; POLYETHYLENEIMINE; POLYELECTROLYTES; POLYSTYRENE; CELL adhesion
- Publication
Polymers (20734360), 2022, Vol 14, Issue 13, p2643
- ISSN
2073-4360
- Publication type
Article
- DOI
10.3390/polym14132643