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- Title
Morphological variability of Indonesian rice germplasm and the associated SNP markers.
- Authors
Lestari, Puji; Utami, Dwinita Wikan; Rosdianti, Ida; Sabran, Muhamad
- Abstract
Morphological and molecular characterization of rice genotypes with varied genetic background is highly considerable to be carried out to their utilization and to genetic variability incorporation into rice breeding program. In this study, the diversity within 60 rice genotypes from different subspecies of indica, japonica and tropical japonica was analyzed for 19 morphological traits and 384-plex SNPs with GoldenGate assay. Principal component analysis (PCA) clustered the germplasm into three main groups (indica, indica/tropical japonica/japonica and indica/tropical japonica). PCA axes 1 and 2 and morphological traits with eigenvalue at arbitrary above 0.2, explained 24.60% of total variation in the rice germplasm. Seven traits (leaf color, leaf sheats color, ligule color, diameter of basal internode, culm color, ligule length, and flag leaf angle) could be the potential traits as descriptors for further characterization and conservation studies in Indonesian rice germplasm. Based on total morphological traits and 384 SNPs, two major clades were generated and differentiated most genotypes into subspecies/cultivar group according to their individual genetic background. Of total 154 SNPs associated with 15 morphological traits, approximately 48 SNPs were highly significant associated with quantitative and quantitative traits. The associated SNPs which were located inside or very near to genes of interest/QTLs would be potential as genetic markers. These alleles could provide breeders to effectively identify, track and incorporate regions of chromosomes governing the desired morphological traits to improve rice varieties in Indonesia.
- Subjects
RICE; MULTIPLE correspondence analysis (Statistics); SINGLE nucleotide polymorphisms; PLANT germplasm; RICE breeders
- Publication
Emirates Journal of Food & Agriculture (EJFA), 2016, Vol 28, Issue 9, p660
- ISSN
2079-052X
- Publication type
Article
- DOI
10.9755/ejfa.2016-03-319