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- Title
Parthenolide induces proliferation inhibition and apoptosis of pancreatic cancer cells in vitro.
- Authors
Jun-Wei Liu; Min-Xia Cai; Ying Xin; Qing-Song Wu; Jun Ma; Po Yang; Hai-Yang Xie; Dong-Sheng Huang
- Abstract
Background: To explore the anti-tumor effects of parthenolide in human pancreatic cancer. Methods: BxPC-3 cell, a human pancreatic cancer, was treated with parthenolide at different concentrations. The MTT assay was used to analyze cell viability. Flow cytometry and DNA fragmentation analysis were applied to evaluate apoptosis after parthenolide treatment. The wound closure and cell invasion assay were also employed in the study. Western blotting was used to demonstrate Bad, Bcl-2, Bax, caspase-9 and pro-caspase-3 expression. Results: The MTT assay indicated that the pancreatic cancer growth could be dose-dependently inhibited by parthenoolide. This phenomenon was confirmed by flow cytometry and DNA fragmentation analysis. The wound closure assay and cell invasion assay showed that BxPC-3 cell was significantly suppressed by parthenolide at 7.5 μM and 15 μM. Western Blotting demonstrated the Bcl-2 and pro-caspase-3 were down-regulated while the Bax and caspase-9 were up-regulated. No alteration in Bad expression was found after treatment. Conclusions: The parthenolide can inhibit the cell growth, migration, and induce the apoptosis in human pancreatic cancer. These findings may provide a novel approach for pancreatic cancer treatment.
- Subjects
CANCER research; PANCREATIC tumors; CANCER treatment; CELL death; CANCER cells; TUMOR growth; DNA; PANCREATIC diseases; MOLECULAR genetics
- Publication
Journal of Experimental & Clinical Cancer Research (17569966), 2010, Vol 29, p108
- ISSN
1756-9966
- Publication type
Article
- DOI
10.1186/1756-9966-29-108