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- Title
P21WAF1/CIP1 messenger RNA expression in hepatitis B, C virus-infected human hepatocellular carcinoma tissues.
- Authors
Kobayashi, Susumu; Matsushita, Kazuyuki; Saigo, Kenichi; Urashima, Tetsuro; Asano, Takehide; Hayashi, Haruyuki; Ochiai, Takenori; Kobayashi, S; Matsushita, K; Saigo, K; Urashima, T; Asano, T; Hayashi, H; Ochiai, T
- Abstract
<bold>Background: </bold>The primary objective of this study was to clarify the significance of p21WAF1/CIP1(p21) gene expression in the tumorgenicity of hepatitis B virus (HBV) and hepatitis C virus (HCV) infected human hepatocelluar carcinoma (HCC).<bold>Methods: </bold>The authors performed Northern blot hybridization to compare the p21 messenger (m) RNA expression levels among 16 HCC cases. They detected tissue HBVx mRNA (Northern blot) and plus- and minus-strand HCV RNA (reverse transcription-polymerase chain reaction) in liver tissues. They also measured alanine transaminase (ALT) levels and indocyanine green retention rate at 15 minutes (ICG-R15).<bold>Results: </bold>The p21 transcripts of tumor (T) tissues could be identified with lower intensity than nontumor (N) tissues in all 4 HBVx mRNA(+) cases, 8 of 10 HCV RNA(+) cases, and 1 of 3 B(-), C(-) cases (1 case was positive for both viruses). p21 mRNA expression levels of N tissues were significantly higher in HCV RNA(+) cases than in HBVx mRNA(+) cases. p21 mRNA expression levels of N tissues were significantly correlated with serum ALT levels.<bold>Conclusions: </bold>In HCV hepatitis, p21 mRNA expression is up-regulated to control cell cycle under regeneration stress. Once the liver develops HCC, the p21 mRNA expression decreases to prominently low levels. The up-regulated p21 expression may play a role as a guard to prevent hepatocytes from tumorgenicity in HCV hepatitis.
- Publication
Cancer (0008543X), 2001, Vol 91, Issue 11, p2096
- ISSN
0008-543X
- Publication type
journal article
- DOI
10.1002/1097-0142(20010601)91:11<2096::AID-CNCR1237>3.0.CO;2-9