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- Title
COMPARATIVE EFFICACY OF SINGLE RADIAL HAEMOLYSIS TEST AND COUNTERCURRENT IMMUNOELECTROOSMO-PHORESIS WITH MONOCLONAL ANTIBODIES-BASED COMPETITIVE ELISA FOR THE SEROLOGY OF PESTE DES PETITS RUMINANTS IN SHEEP AND GOATS.
- Authors
Munir, M.; Abubakar, M.; Khan, M. T.; Abro, S. H.
- Abstract
This project was conducted to investigate the comparative efficiency of competitive ELISA (cELISA), single radial haemolysis test (SRH) and countercurrent immunoelectroosmophoresis (CIEOP) for the diagnosis of Peste des Petits Ruminants (PPR) in sheep and goats. Serum samples from 198 sheep and 82 goats were collected from three different government livestock farms and all the samples were run simultaneously with three serological tests. The samples found positive for PPR antibodies through cELISA, SRH and CIEOP were 96 (34.2%), 55 (19.6%) and 67 (23.9%), respectively. Kappa statistics was used to evaluate the concordance between the laboratory-based test (cELISA) and field-based tests (SRH and CIEOP). Kappa statistics scores for cELISA vs SRH and CIEOP were 0.5851 (95% confidence interval 0.4848-0.6854) and 0.6668 (95% confidence interval 0.5733-0.7603), respectively, which indicate a "moderate" agreement between cELISA and SRH and "substantial" agreement between cELISA and CIEOP. SRH and CIEOP revealed a relative diagnostic sensitivity with cELISA of 54.1% and 64.5% and relative diagnostic specificity of 98.3% and 97.2%, respectively. The data suggested that for mass screening and control of PPR, these serological tests proved practical in the absence of cELISA since they have a high relative diagnostic specificity and a satisfactory relative diagnostic sensitivity.
- Subjects
HEMOLYTIC plaque technique; IMMUNOELECTROPHORESIS; ENZYME-linked immunosorbent assay; CONFIDENCE intervals; MEDICAL screening
- Publication
Bulgarian Journal of Veterinary Medicine, 2009, Vol 12, Issue 4, p246
- ISSN
1311-1477
- Publication type
Article