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- Title
Protein Phosphatase 1-Targeting Small-Molecule C31 Inhibits Ebola Virus Replication.
- Authors
Ammosova, Tatiana; Pietzsch, Colette A; Saygideğer, Yasemin; Ilatovsky, Andrey; Lin, Xionghao; Ivanov, Andrey; Kumari, Namita; Jerebtsova, Marina; Kulkarni, Amol; Petukhov, Michael; Saygideger, Yasemin; Üren, Aykut; Bukreyev, Alexander; Nekhai, Sergei
- Abstract
<bold>Background: </bold>Ebola virus (EBOV) infection causes severe hemorrhagic fever. EBOV transcription is controlled by host protein phosphatase 1 (PP1), which dephosphorylates VP30 protein. We previously developed 1E7-03, a compound targeting a noncatalytic site of PP1 that induced VP30 phosphorylation and inhibited EBOV transcription. Here, we attempted to further improve 1E7-03, which was not stable in murine serum.<bold>Results: </bold>High-throughput screening with EBOV-green fluorescent protein was conducted on 72 1E7-03 analogs and identified 6 best inhibitory and the least toxic compounds. A parallel in silico screening of compounds from the ZINC database by docking to PP1 identified the best-binding compound C31, which was also present among the top 6 compounds found in the viral screen. C31 showed the best EBOV inhibitory activity among the top 6 compounds and also inhibited EBOV minigenome. C31 bound to the PP1 C-terminal groove in vitro and increased VP30 phosphorylation in cultured cells. C31 demonstrated improved stability in mouse plasma and cell permeability, compared with 1E7-03. It was also detected for 24 hours after injection in mice.<bold>Conclusion: </bold>C31 represents a novel PP1-targeting EBOV inhibitor with improved pharmacological properties that can be further evaluated for future antifiloviral therapy.
- Subjects
EBOLA virus disease; HEMORRHAGIC fever; PHOSPHOPROTEIN phosphatases; DEPHOSPHORYLATION; CELL permeability
- Publication
Journal of Infectious Diseases, 2018, Vol 218, pS627
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiy422