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- Title
Effect of Neurotrophin-4/5 on Bone/ Cementum-Related Protein Expressions and DNA Synthesis in Cultures of Human Periodontal Ligament Cells.
- Authors
Mizuno, Noriyoshi; Shiba, Hideki; Inui, Takafumi; Takeda, Katsuhiro; Kajiya, Mikihito; Hasegawa, Naohiko; Kawaguchi, Hiroyuki; Kurihara, Hidemi
- Abstract
Background: We studied neurotrophins (NTs) as signaling molecules for periodontal tissue regeneration and showed that nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) modulate the proliferation and differentiation of human periodontal ligament (HPL) cells in vitro. The purpose of this study was to investigate whether NT-4/5 also has the ability to regulate the function of HPL cells. Methods: mRNA expressions of NT-4/5 and its high-affinity tyrosine kinase receptor (trk)B were analyzed in HPL cells by reverse transcription-polymerase chain reaction. We examined how NT-4/5 regulates the mRNA expression of bone/ cementum-related proteins (alkaline phosphatase [ALPase], osteopontin [OPN], osteocalcin [OC], and bone morphogenetic protein [BMP]-2) in cultures of HPL cells. Moreover, the effects of NT-4/5 on calcification, the production of OPN and OC, and DNA synthesis in HPL cells were examined, Results: NT-4/5 and trkB mRNA were expressed in HPL cells. NT-4/5 elevated the mRNA levels of ALPase, OPN, OC, and BMP 2 and the syntheses of OPN, OC, and DNA in HPL cells. PD98059, an extracellular signal-regulated kinase (ERK) inhibitor, obviated the increase in the mRNA levels of ALPase, OPN, OC, and BMP-2. NT-4/5 increased the levels of phosphorylated ERK1/2. Furthermore, NT-4/5 enhanced the amount of mineral deposits in cultures of HPL cells. Conclusion: NT-4/5, as well as BDNF and NGF, is suggested to play a role in the regulation of function of periodontal ligament cells.
- Subjects
GUIDED tissue regeneration; NERVE growth factor; PERIODONTAL ligament; CELLS; MESSENGER RNA; REVERSE transcriptase polymerase chain reaction
- Publication
Journal of Periodontology, 2008, Vol 79, Issue 11, p2182
- ISSN
0022-3492
- Publication type
Article
- DOI
10.1902/jop.2008.070402