We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Functional effects of cytoskeletal components on the lateral movement of individual BK<sub>Ca</sub> channels expressed in live COS-7 cell membrane
- Authors
Won, Sehoon; Lee, Byoung-Cheol; Park, Chul-Seung
- Abstract
Abstract: The lateral diffusion of BKCa channels was previously shown to be highly ‘confined’ in the COS-7 cell membrane. Here we report that the diffusion coefficient and the confinement area of BKCa channel were significantly increased by the treatment of latrunculin A, an actin-depolymerizing agent, but not by microtubule disruption. Site-directed mutational analyses further demonstrated that a single leucine residue in the C-terminal actin-binding motif was critical for the aforementioned effects of latrunculin A. We conclude that some BKCa channels are directly associated with actin filaments and their lateral mobility can be restricted by the cytoskeletal components.
- Subjects
CELL membranes; CALCIUM channels; CYTOSKELETAL proteins; PEPTIDES; DIFFUSION; QUANTUM dots; TOXINS; MICROTUBULES
- Publication
FEBS Letters, 2011, Vol 585, Issue 14, p2323
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.05.069