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- Title
Recovery of functional enzyme from amyloid fibrils
- Authors
Agócs, Gergely; Solymosi, Katalin; Varga, Andrea; Módos, Károly; Kellermayer, Miklós; Závodszky, Péter; Fidy, Judit; Osváth, Szabolcs
- Abstract
Abstract: Amyloid deposits, which accumulate in numerous diseases, are the final stage of multi-step protein conformational-conversion and oligomerization processes. The underlying molecular mechanisms are not fully understood, and particularly little is known about the reverse reaction. Here we show that phosphoglycerate kinase amyloid fibrils can be converted back into native protein. We achieved recovery with 60% efficiency, which is comparable to the success rate of the unfolding-refolding studies, and the recovered enzyme was folded, stable and fully active. The key intermediate stages in the recovery process are fibril disassembly and unfolding followed by spontaneous protein folding.
- Subjects
AMYLOID beta-protein; MOLECULAR biology; PROTEIN kinases; ENZYMATIC analysis; PROTEIN folding; CONFORMATIONAL analysis
- Publication
FEBS Letters, 2010, Vol 584, Issue 6, p1139
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2010.01.058