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- Title
N9L and L9N mutations toggle Hha binding and hemolysin regulation by Escherichia coli and Vibrio cholerae H-NS
- Authors
García, Jesús; Madrid, Cristina; Cendra, Mar; Juárez, Antonio; Pons, Miquel
- Abstract
Abstract: Proteins of the Hha/YmoA family co-regulate with H-NS the expression of virulence factors in Enterobacteriaceae. Vibrio cholerae lacks Hha-like proteins and its H-NS (vcH-NS) is unable to bind Hha, in spite of the conservation of a key residue for Hha binding by Escherichia coli H-NS (ecH-NS). Exchange of the residues in position 9 between vcH-NS and ecH-NS strongly reduces Hha binding by ecH-NS and introduces it in vcH-NS. These mutations strongly affect the repression of the hemolysin operon in E. coli and the electrophoretic mobility of complexes formed with a DNA fragment containing its regulatory region.
- Subjects
GENETIC mutation; ETHYLENEDIAMINE; ACETIC acid; ESCHERICHIA coli; GENETIC regulation; PROTEIN binding; VIBRIO cholerae; MICROBIAL virulence
- Publication
FEBS Letters, 2009, Vol 583, Issue 17, p2911
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.07.054