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- Title
A novel ferritin gene, SferH-5, reveals heterogeneity of the 26.5-kDa subunit of soybean (Glycine max) seed ferritin
- Authors
Dong, Xiangbai; Sun, Qiming; Wei, Dongping; Li, Jiahuang; Li, Jie; Tang, Bo; Jia, Qi; Hu, Wenjun; Zhao, Yongjuan; Hua, Zi-Chun
- Abstract
Abstract: A novel ferritin cDNA, SferH-5, has been cloned from 7-day-old soybean seedlings. Putative SferH-5 has 96% identity with SferH-1 reported previously. All the five amino acid variants distributed in the mature region are not involved in highly conserved residues associated with ferroxidase activity center. We speculate that SferH-5 encodes a novel 26.5-kDa subunit of soybean seed ferritin, which is designated H-5 in this study. Recombinant H-5 was able to assemble, together with co-expressed H-2, as a functional soybean seed ferritin-like complex, H-5/H-2. Our data reveal the potential heterogeneity of the 26.5-kDa subunit of soybean seed ferritin.
- Subjects
FERRITIN; SOYBEAN; MOLECULAR cloning; AMINO acids
- Publication
FEBS Letters, 2007, Vol 581, Issue 30, p5796
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.11.049