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- Title
Expression of bone morphogenetic proteins in human osteosarcoma. Immunohistochemical detection with monoclonal antibody.
- Authors
Yoshikawa, Hideki; Rettig, Wolfgang J.; Takaoka, Kunio; Alderman, Edward; Rup, Bonita; Rosen, Vicki; Wozney, John M.; Lane, Joseph M.; Huvos, Andrew G.; Garin-Chesa, Pilar; Yoshikawa, H; Rettig, W J; Takaoka, K; Alderman, E; Rup, B; Rosen, V; Wozney, J M; Lane, J M; Huvos, A G; Garin-Chesa, P
- Abstract
<bold>Background: </bold>Bone morphogenetic proteins (BMP) induce ectopic bone formation in vivo and may play a role in normal bone development. In addition, bone morphogenetic activity, as measured in a bone-forming assay in immunodeficient, athymic nu/nu mice, is present in a proportion of osteosarcomas; this activity, which may be mediated by BMP, is correlated with a poor prognosis.<bold>Methods: </bold>The development of a monoclonal antibody against recombinant human BMP-2, AbH3b2/17, has allowed immunohistochemical localization of BMP in tumor tissues. Cryostat sections of osteosarcomas (21 tumor samples), chondrosarcomas (5 samples), and Ewing's sarcomas of bone (5 samples) were examined with AbH3b2/17 using the avidin-biotin-immunoperoxidase method.<bold>Results: </bold>The authors found AbH3b2/17 immunoreactivity in 12 of the 21 osteosarcoma samples (57% sensitivity) obtained from 20 patients. For one patient, samples of the primary lesion and a subsequent metastasis were tested, and only the latter showed AbH3b2/17 immunoreactivity. The chondrosarcomas and Ewing's sarcomas examined showed no immunoreactivity. In antigen-positive osteosarcomas, AbH3b2/17 immunostaining was localized predominantly in the cytoplasm of tumor cells. Moreover, the proportion of AbH3b2/17-reactive cells varied among osteosarcomas with disparate histologic features.<bold>Conclusions: </bold>The authors identified a rapid and widely applicable method for detecting BMP expression in intact tissues, which may complement and enhance the bone-forming assay in nu/nu mice as a prognostic procedure in osteosarcomas.
- Publication
Cancer (0008543X), 1994, Vol 73, Issue 1, p85
- ISSN
0008-543X
- Publication type
journal article
- DOI
10.1002/1097-0142(19940101)73:1<85::AID-CNCR2820730116>3.0.CO;2-8