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- Title
Permeation of polysucrose 15 000 across the human nasal mucosa <em>in vivo</em>.
- Authors
Andersson, M.; Greiff, L.; Öman, H.; Svensson, C.; Wollmer, P.; Persson, C. G. A.
- Abstract
Studies of tile nasal permeation of small molecules (< 1000 Da) have yielded important information about the integrity of the human airway mucosa in health and disease. In this study, we used a much larger tracer molecule, polysucrose (PS) 15 000 (approx. 14700 Da), to predict the mucosal permeation of inhalational allergens. PS 15 000 (50 mg/ml; 15 ml), with or without a detergent type of permeation enhancer (dioctyl sodium sulfosuccinate 10 mg/ml), was maintained for 15 min in one nasal cavity of 12 healthy nonatopic subjects by employment of a nasal-pool device. Permeation as determined by the 24-h urine recovery of PS (micro-ELISA analysis assay) was expressed as percentage of nasal instillate. Mean baseline permeation was 0.044% (range 0.009-0.250%). In the presence of the detergent, permeation increased to 0.600% (range 0.007-2.260%) (<em>P</em> < 0.01). After oral intake of 750 mg of PS 15 000 (50 μg/ml: 15 ml), the 24-h urinary recovery was 0.013% (range 0.004-0.023%). Our study thus demonstrates a measurable baseline permeation of PS 15 000, an elevated permeation rate in the presence of an epithelium-damaging detergent molecule, and a negligible permeation by the oral route. These properties support the utility of PS 15 000 as a nasal airway permeation tracer. Its size further suggests that its permeation may reflect mucosal perviousness to many allergens.
- Subjects
ALLERGENS; MUCOUS membranes; NASAL cavity; INFLAMMATION; EPITHELIUM; MOLECULES
- Publication
Allergy, 1997, Vol 52, Issue 12, p1229
- ISSN
0105-4538
- Publication type
Article
- DOI
10.1111/j.1398-9995.1997.tb02528.x