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- Title
Focused DNA microarray analysis for sex-dependent gene expression of drug metabolizing enzymes, transporters and nuclear receptors in rat livers and kidneys.
- Authors
Shunsuke Iwano; Eriko Higashi; Tomoya Miyoshi; Akihiro Ando; Yohei Miyamoto
- Abstract
Cytochrome P450(CYP)s are known to show a sexual dimorphic expression in rat livers. However, the comprehensive analysis for the sex-dependent gene expressions of drug metabolizing enzymes except for CYPs, transporters and nuclear receptors in rat livers and kidneys has not been investigated yet. The purpose of the present study was to identify the novel drug metabolizing and pharmacokinetics (DMPK)-related gene(s) which show the sex difference in the mRNA expressions in rat livers and kidneys. Total RNAs were prepared from livers and kidneys in both male and female rats (Crl:CD(SD) and Crlj:WI). A DNA microarray analysis using a "GeneSQUARE Multiple Assay DNA Microarray Drug Metabolism Gene Expression for Rat" was performed. DMPK-related genes which showed sex differences in the mRNA expression were identified in rat livers or kidneys. Especially, the female dominant expressions of UDP glucuronosyltransferase (UGT) s were seen in rat livers and kidneys. The sex difference of UGT expressions in rats might be one of the causal factors of the sex difference of the biological response to UGT substrates.
- Subjects
DNA microarrays; GENE expression; SEX differences (Biology); DRUG metabolism; NUCLEAR receptors (Biochemistry); CYTOCHROME P-450; LABORATORY rats
- Publication
Journal of Toxicological Sciences, 2012, Vol 37, Issue 4, p863
- ISSN
0388-1350
- Publication type
Article
- DOI
10.2131/jts.37.863