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- Title
α-淀粉酶及其复合酶体系对淀粉酶解 性能研究.
- Authors
朱鹤馨; 汪思维; 胡慧捷; 赵 铭; 刘 念; 胡 静; 蒋 云; 邓海波
- Abstract
This study investigated the enzymatic performance of single α-amylase and binary, ternary, and quaternary complex enzyme systems consisting of α-amylase with β-amylase, pullulanase and glucoamylase for three commonly used modified starches (oxidized starch, enzymatically hydrolyzed starch, and cationic starch). The effect of different α-amylase dosages on starch enzymatic rate and DE value was explored. The results indicated that when the starch enzymatic rate reached 90% in single α-amylase system, the required amount of α-amylase for cationic starch enzymolysis was about 4 times than that of the other two kinds of starches. The enzymatic rate of enzymatically hydrolyzed starch could be increased by up to 37. 4% when citric acid was added to inhibit zinc ions. In the binary complex enzyme system, the enzymatic rate of cationic starch by α-amylase/β-amylase was 5. 3% and 8. 7% higher than that by α-amylase/glucoamylase and α-amylase/pullulanase, respectively. In the ternary complex enzyme system, the enzymatic rate of cationic starch by α-amylase/glucoamylase/β -amylase was 1. 2% higher than that by α-amylase/glucoamylase/pullulanase. In the application of α-amylase/glucoamylase/β-amylase/pullulanase tetrameric complex enzyme system to the enzymatic reactions of cationic starch, enzymatically hydrolyzed starch and oxidized starch, when the enzymatic rate of starch reached 80%, the required amount of α-amylase was 56%, 38% and 17% of that by single α-amylase system, respectively.
- Publication
Transactions of China Pulp & Paper, 2024, Vol 39, Issue 3, p87
- ISSN
1000-6842
- Publication type
Article
- DOI
10.11981/j.issn.1000‐6842.2024.03.87