We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
DEVELOPMENT OF THE LACTIC ACID PERMEABILIZER v-qPCR TECHNIQUE FOR Salmonella spp. DETECTION AND QUANTIFICATION.
- Authors
AL-ZAIDI, Quthama; MATACHE, Adelina Georgiana; DINU, Laura-Dorina; CALZONI, Eleonora; MATEI, Florentina
- Abstract
In the food industry, one of the biggest challenges is microbial contamination. Viability real-time PCR (v-qPCR) technology has made the detection and quantification of pathogens easier, and some issues remain with the accuracy of the results, especially in samples containing viable and non-viable cells. V-qPCR with propidium monoazide (PMA) and pre-treatment with permeability agents is one promising method to circumvent this issue. Therefore, the aim of the study was to improve the Salmonella spp. detection using a method with lactic acid (LA 5-10 mM) pre-treatment that permeabilized Gram-negative outer membrane. The signal reduction (dCt = 13.97) after 10 mM LA pre-treatment was similar with the reduction in Ct signal (dCt = 13.62) when only PMA treatment was applied to a suspension of nonviable cells. In the case of viable cells, the LA pre-treatment improved the v-qPCR detection. These preliminary studies provide useful information on the use of lactic acid, which has proven to be an effective, cheap and low-toxic permeabilizing agent.
- Publication
AgroLife Scientific Journal, 2024, Vol 13, Issue 1, p9
- ISSN
2285-5718
- Publication type
Article