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- Title
Molecular and biochemical characteristics of inulosucrase InuBK from Alkalihalobacillus krulwichiae JCM 11691.
- Authors
Yokoi, Ken-ji; Tsutsui, Sosyu; Arakawa, Gen-ya; Takaba, Masakazu; Fujii, Koichi; Kaneko, Satoshi
- Abstract
Information about the inulosucrase of nonlactic acid bacteria is scarce. We found a gene encoding inulosucrase (inuBK) in the genome of the Gram-positive bacterium Alkalihalobacillus krulwichiae JCM 11691. The inuBK open reading frame encoded a protein comprising 456 amino acids. We expressed His-tagged InuBK in culture medium using a Brevibacillus system. The optimal pH and temperature of purified InuBK were 7.0-9.0 and 50-55 °C, respectively. The findings of high-performance anion-exchange chromatography, nuclear magnetic resonance spectroscopy, and high-performance size-exclusion chromatography with multiangle laser light scattering showed that the polysaccharide produced by InuBK was an inulin with a molecular weight of 3806, a polydispersity index (PI) of 1.047, and fructosyl chain lengths with 3-27 degrees of polymerization. The size of InuBK was smaller than commercial inulins, and the PI of the inulin that it produced was lower.
- Subjects
INULIN; DEGREE of polymerization; POLYSACCHARIDES; NUCLEAR magnetic resonance spectroscopy
- Publication
Bioscience, Biotechnology & Biochemistry, 2021, Vol 85, Issue 8, p1830
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbab094