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- Title
Monitoring ADP/ATP ratio in yeast cells using the fluorescent-protein reporter PercevalHR.
- Authors
Nguyen, Phuong Thi Mai; Ishiwata-Kimata, Yuki; Kimata, Yukio
- Abstract
PercevalHR (Perceval High Resolution) is an artificially designed fluorescent protein, which changes its excitation spectrum based on the ADP/ATP ratio of the environment. Here we demonstrated that PercevalHR can be used for monitoring energy status of Saccharomyces cerevisiae cells, which are affected by diauxic shift and mitochondria inhibition, in a non-invasive and non-destructive manner. Yeast cells producing PercevalHR emit green fluorescence, excitation spectrum of which varies dependently on cellular ATP/ATP ratio.
- Subjects
ADENOSINE diphosphate; YEAST; FLUORESCENT proteins
- Publication
Bioscience, Biotechnology & Biochemistry, 2019, Vol 83, Issue 5, p824
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2019.1574204