We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Intracellular localization of sirtuin and cell length analysis of <italic>Lactobacillus paracasei</italic> suggest possible role of sirtuin in cell division and cell shape regulation.
- Authors
Torres-Barredo, Glaezel Angelique; Atarashi, Hotaka; Kajikawa, Akinobu; Hirata, Aiko; Endo, Akihito; Nakagawa, Junichi
- Abstract
Sirtuin has been associated in prolonging lifespan of different model organisms. It has been shown to have an enzymatic activity of NAD+-dependent protein deacetylation targeting acetylated proteins. To determine targets and possible roles of sirtuin (LpSirA) in the <italic>Lactobacillus paracasei</italic> BL23, deletion (Δ<italic>sirA</italic>), sirtuin overexpressor (high<italic>sirA</italic>) and GFP fusion (high<italic>sirA</italic>-Venus) strains were generated, and microscopic localization and cell length analysis were done. Microscopic analysis revealed localization of LpSirA at cell division plates, at cell poles and all throughout the cell length in a spiral manner. Cell length analysis revealed that 46.9% of the Δ<italic>sirA</italic> cells were observed to be shorter (<2 μm), whereas 12.6% of the high<italic>sirA</italic> cells were observed to be longer (>4 μm) in comparison with the wild-type with only 17.1% short cells and 5.3% long cells. Our results suggest that sirtuin may have an essential role in cell division and cell shape regulation.
- Subjects
LACTOBACILLUS; SIRTUINS; NAD (Coenzyme)
- Publication
Bioscience, Biotechnology & Biochemistry, 2018, Vol 82, Issue 5, p916
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2018.1443790