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- Title
Comparative effects of ethylene inhibitors on <italic>Agrobacterium</italic>-mediated transformation of drought-tolerant wild watermelon.
- Authors
Malambane, Goitseone; Nonaka, Satoko; Shiba, Hiroshi; Ezura, Hiroshi; Tsujimoto, Hisashi; Akashi, Kinya
- Abstract
Ethylene (C2H4), a phytohormone that is produced in response to both abiotic and biotic stresses, is an important factor influencing the efficiency of <italic>Agrobacterium</italic>-mediated transformation. In this study, effects of various ethylene inhibitors on the efficiency of <italic>Agrobacterium</italic>-mediated genetic transformation in drought-tolerant wild watermelon was comparatively examined. Consequently, in comparison to the application of chemical inhibitors such as AgNO3 and aminoethoxyvinylglycine (AVG), lower ethylene level was observed when the infecting <italic>Agrobacterium</italic> contained a gene for 1-aminocyclopropane-carboxylic acid (ACC) deaminase (<italic>acdS</italic>), which cleaves ethylene precursor ACC into α-ketobutyrate and ammonia. GUS histochemical and spectrophotometric enzyme assays showed that <italic>acdS</italic> was more effective in enhancing gene transfer than the chemical ethylene inhibitors. Efficiency of transgenic shoots formation was higher in <italic>acdS</italic>- and AVG-treated explants. These observations demonstrated that controlling the ethylene level during co-cultivation and shoot formation, particularly using the <italic>acdS</italic>-harboring <italic>Agrobacterium,</italic> is advantageous for enhancing the transformation efficiency in this plant.
- Subjects
ETHYLENE; WATERMELONS; AGROBACTERIUM
- Publication
Bioscience, Biotechnology & Biochemistry, 2018, Vol 82, Issue 3, p433
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2018.1431516