We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Polymeric proteins and their association with grain yield in hard red spring wheat lines.
- Authors
Tsilo, Toi; Hareland, Gary; Ohm, Jae-Bom; Anderson, James
- Abstract
Development of high yielding wheat ( Triticum aestivum L.) varieties with acceptable end-use quality is a major focus in breeding programs worldwide. Variations in molecular weight (Mw) distribution of endosperm proteins are known to influence end-use quality traits. This paper reports the relationship of the size-exclusion high performance liquid chromatography (SE-HPLC) profile of endosperm proteins with grain yield. Flour samples were previously analyzed for Mw distribution of sodium-dodecyl sulfate (SDS) extractable and unextractable proteins using the SE-HPLC protocol. Correlations were calculated between grain yield and HPLC absorbance data obtained at 0.01-min retention time intervals. Although both SDS-extractable and unextractable proteins had positive correlations with grain protein content, only SDS-unextractable very high Mw polymeric proteins (UVHP) had no negative association with grain yield, while SDS-extractable fractions rich in low Mw polymeric proteins had a negative correlation ( r = −0.41) with grain yield. These results suggest that in an effort to increase grain yield, breeding programs should target grain yield and also increase levels of UVHP and decrease SDS-extractable polymeric proteins, thereby, maintaining acceptable bread-making quality.
- Subjects
WHEAT genetics; HIGH performance liquid chromatography; WHEAT varieties; RED spring wheat; WHEAT breeding; WHEAT yields
- Publication
Euphytica, 2013, Vol 194, Issue 2, p187
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-013-0912-4