We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Metabolite detection in Rhodococcus benzothiophene desulfurization using reversed-phase solid-phase extraction and a derivatization technique.
- Authors
Onaka, Toshimitsu; Matsui, Toru; Maruhashi, Kenji
- Abstract
Hydrophilic benzo[b]thiophene metabolites produced by Rhodococcus sp. strain T09 were isolated by a solid-phase extraction and a derivatization method, which prevented the dehydration that often occurs during liquid-liquid extraction (LLE), and were identified by GC with atomic emission detection and GC-MS. As a result, cis- or trans-2-(2′-hydroxyphenyl) ethen-1-ol, previously reported as the tautomer 2-(2′-hydroxyphenyl) ethan-1-al, and 2-(2′-hydroxyphenyl) ethen-1-sulfenic acid and its isomer, 1-(1′-hydroxyethenyl) benzene-2-sulfenic acid, were identified. Benzothiophene desulfurization in culture broth at neutral pH by the strain T09 was modified as a branched metabolism from benzothiophene S-oxide based on the above compounds.
- Subjects
MICROBIAL metabolites; AROMATIC compounds; DERIVATIZATION; SOLID phase extraction; SOLID-phase biochemistry; ATOMIC emission spectroscopy
- Publication
Biotechnology Letters, 2002, Vol 24, Issue 23, p1961
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1021154528924