We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A New Type of Nitrogen-fixing Bacterium.
- Authors
ROY, A. B.; MUKHERJEE, M. K.
- Abstract
IN the course of an investigation in the Jute Agricultural Research Institute on nitrogen transformation in soils cropped with jute, a nitrogen-fixing bacterium has been isolated by the dilution-plate technique. It shows certain morphological and physiological differences from the known non-symbiotic nitrogen-fixing organisms like Pseudomonas azotocalligans and those belonging to the Azotobacteriaceae1. The cells at first have a coccus-like appearance and occur singly (measuring 0.75-1.0 × 1.5-2.5µ on an average) or as doublets or triplets, gradually changing to rod shape (Fig. 1). The cells are Gram-negative; they contain fat and are very difficult to suspend in water. The optimum temperature for growth was found to be about 28° C. The organism is aerobic, but can be grown under low oxygen tension in Winogradsky's nitrogen-free glucose-phosphate media. It can grow in acidic media also; even at pH 4.0 growth was obtained. Bromo-cresol purple milk (skimmed) is turned alkaline and a sediment is formed at the bottom with a clear liquid surface after ten days incubation at 30° C.
- Publication
Nature, 1957, Vol 180, Issue 4579, p236
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/180236a0