We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Isolation and characterization of a phenol-degrading Rhodococcus sp. strain AQ5NOL 2 KCTC 11961BP.
- Authors
Arif, N. M.; Ahmad, S. A.; Syed, M. A.; Shukor, M. Y.
- Abstract
In this work, we report on the isolation of a phenol-degrading Rhodococcus sp. with a high tolerance towards phenol. The isolate was identified as Rhodococcus sp. strain AQ5NOL 2, based on 16S rDNA analysis. The strain degraded phenol using the meta pathway, a trait shared by many phenol-degraders. In addition to phenol biodegradation, the strain was also capable of degrading diesel. Strain AQ5NOL 2 exhibited a broad optimum temperature for growth on phenol at between 20 °C and 35 °C. The best nitrogen sources were ammonium sulphate, glycine or phenylalanine, followed by proline, nitrate, leucine, and alanine (in decreasing efficiency). Strain AQ5NOL 2 showed a high tolerance and degradation capacity of phenol, for it was able to register growth in the presence of 2000 mg l−1 phenol. The growth of this strain on phenol as sole carbon and energy source were modeled using Haldane kinetics with a maximal specific growth rate ( μmax) of 0.1102 hr−1, a half-saturation constant ( K s) of 99.03 mg l−1 or 1.05 mmol l−1, and a substrate inhibition constant ( K i) of 354 mg l−1 or 3.76 mmol l−1. Aside from phenol, the strain could utilize diesel, 2,4-dinitrophenol and ρ-cresol as carbon sources for growth. Strain AQ5NOL 2 exhibited inhibition of phenol degradation by Zn2+, Cu2+, Cr6+, Ag+ and Hg2+ at 1 mg l−1.
- Subjects
RHODOCOCCUS; NOCARDIACEAE; PHENOLS; DNA; NUCLEIC acids; AMMONIUM sulfate
- Publication
Journal of Basic Microbiology, 2013, Vol 53, Issue 1, p9
- ISSN
0233-111X
- Publication type
Article
- DOI
10.1002/jobm.201100120