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- Title
Trypsin inhibitor from Leucaena leucocephala seeds delays and disrupts the development of Aedes aegypti, a multiple-disease vector.
- Authors
Almeida Filho, Luiz CP; de Souza, Terezinha M; Tabosa, Pedro MS; Soares, Nayana G; Rocha‐Bezerra, Lady CB; Vasconcelos, Ilka M; Carvalho, Ana FU
- Abstract
BACKGROUND Nowadays, the Aedes aegypti mosquito represents a serious public health issue in view of the large outbreaks of the arboviral diseases zika, dengue, chikungunya and yellow fever. This holometabolous insect has midgut digestive enzymes that are trypsin- and chymotrypsin-like proteins. Protease inhibitors are able to bind to proteolytic enzymes and promote a blockage in digestion and nutrition, leading to death. Thus, we investigated the effect of trypsin inhibitor of Leucaena leucocephala ( LTI) seeds on egg hatching, larval development and digestive midgut proteases. RESULTS LTI was obtained by trichloroacetic acid precipitation followed by a single chromatography step on anhydrous trypsin sepharose. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate showed a single protein band with a molecular mass close to 20 kDa. After exposure of Ae. aegypti eggs to LTI (0.3 mg mL−1), egg hatching was reduced (50%). LTI did not show acute toxicity on newly hatched larvae incubated under the same conditions, but after 10 days of exposure a high mortality rate (86%) was observed and the surviving larvae had a 25% delay in development. LTI was able to inhibit in vitro the midgut enzymatic activity (70%), and when larvae were incubated with LTI solution we observed an inhibition of 56%. CONCLUSIONS LTI is a promising new tool to control critical points of Ae. aegypti development. © 2016 Society of Chemical Industry
- Subjects
LEAD tree; TRYPSIN inhibitors; COMPOSITION of seeds; AEDES aegypti; BIOLOGICAL control of mosquitoes
- Publication
Pest Management Science, 2017, Vol 73, Issue 1, p181
- ISSN
1526-498X
- Publication type
Article
- DOI
10.1002/ps.4284