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- Title
Syndecan-4 functionalization of tissue regeneration scaffolds improves interaction with endothelial progenitor cells.
- Authors
Warner, Harleigh; Wu, Yidi; Wagner, William D
- Abstract
Key to most implanted cell free scaffolds for tissue regeneration is the ability to sequester and retain undifferentiated mesenchymal stem cells at the repair site. In this report, syndecan-4, a heparan sulfate containing proteoglycan, was investigated as a unique molecule for use in scaffold functionalization. An electrospun hybrid scaffold comprised of poly (glycerol) sebacate (PGS), silk fibroin and type I collagen (PFC) was used as a model scaffold to develop a procedure and test the hypothesis that functionalization would result in increased scaffold binding of endothelial progenitor cells (EPCs). For these studies both Syndecan-4 and stromal derived factor-1α (SDF-1α) were used in functionalization PFC. Syndecan-4 functionalized PFC bound 4.8 fold more SDF-1α compared to nonfunctionalized PFC. Binding was specific as determined by heparin displacement studies. After culture for 7 days, significantly, more EPCs were detected on PFC scaffolds having both syndecan-4 and SDF-1α compared to scaffolds of PFC with only syndecan-4, or PFC adsorbed with SDF-1α, or PFC alone. Taken together, this study demonstrates that EPCs can be bound to and significantly expanded on PFC material through syndecan-4 mediated growth factor binding. Syndecan-4 with a multiplicity of binding sites has the potential to functionalize and expand stem cells on a variety of scaffold materials for use in tissue regeneration.
- Subjects
PROGENITOR cells; ENDOTHELIAL cells; MESENCHYMAL stem cells; STEM cells; TISSUE scaffolds; HEPARAN sulfate; POLYCAPROLACTONE
- Publication
Regenerative Biomaterials, 2021, Vol 8, Issue 6, p1
- ISSN
2056-3418
- Publication type
Article
- DOI
10.1093/rb/rbab070