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- Title
实时定量PCR评估金针菇的内参基因稳定性.
- Authors
贾定洪; 王波
- Abstract
Flammulina filiformis is one of the most popular edible mushrooms in China and Japan, ranking fourth among the most important cultivated mushrooms according to its production and consumption. Its active constituents, molecular markers, gene identification, etc. have been extensively studied, however, nothing has been reported about its reference-gene stabilities, resulting that there is no available data concerned in the study of gene expression. In this study, 18 S ribosomal RNA(18 S), 28 S ribosomal RNA(28 S), 60 S ribosomal protein L18(Rpl18), actin 1(Act1), glyceraldehyde-3-phosphate dehydrogenase(Gapdh), translation elongation factor EF1-alpha(Ef1 A), DNA-direct RNA polymerase subunit 2(Rpb2), cytochrome c oxidase subunit 1(Cox1) and cytochrome b(CytB) were employed as reference-gene candidates to evaluate their stabilities using algorithms of geNorm, NormFinder, BestKeeper and RefFinder. Comparative results showed that, as a single gene, Act1 was the most stable reference gene, and CytB was the least stable, but a single reference gene was inadequate for normalization in RT-qPCR. Average expression stability values M generated by geNorm indicated that CytB and Rpb2 were the most stable genes and could be taken as suitable reference gene set. This study first evaluated reference-gene stabilities in F. filiformis, and might provide reference for gene expression analysis of this species.
- Subjects
JAPAN; CHINA; CYTOCHROME oxidase; RNA polymerase II; CYTOCHROME b; RIBOSOMAL proteins; RNA polymerases; RIBOSOMAL RNA; RIBOSOMAL DNA
- Publication
Mycosystema, 2021, Vol 40, Issue 7, p1700
- ISSN
1672-6472
- Publication type
Article