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- Title
Comprehensive miRNA Profiling of Skeletal Muscle and Serum in Induced and Normal Mouse Muscle Atrophy During Aging.
- Authors
Hwa Jin Jung; Kwang-Pyo Lee; Milholland, Brandon; Yeo Jin Shin; Jae Sook Kang; Ki-Sun Kwon; Yousin Suh; Jung, Hwa Jin; Lee, Kwang-Pyo; Shin, Yeo Jin; Kang, Jae Sook; Kwon, Ki-Sun; Suh, Yousin
- Abstract
Age-associated loss of muscle mass and function is a major cause of morbidity and mortality in the elderly adults. Muscular atrophy can also be induced by disuse associated with long-term bed rest or disease. Although miRNAs regulate muscle growth, regeneration, and aging, their potential role in acute muscle atrophy is poorly understood. Furthermore, alterations in circulating miRNA levels have been shown to occur during aging but their potential as noninvasive biomarkers for muscle atrophy remains largely unexplored. Here, we report comprehensive miRNA expression profiles by miRNA-seq analysis in tibialis anterior muscle and serum of a disuse-induced atrophy mouse model, mimicking the acute atrophy following long-term bed rest, as compared to those of young and old mice. Comparative analysis and validation studies have revealed that miR-455-3p was significantly decreased in muscle of both induced-atrophy model and old mice, whereas miR-434-3p was decreased in both serum and muscle of old mice, as compared to young mice. Furthermore, upregulation of miR-455-3p in fully differentiated C2C12 myoblasts induced a hypertrophic phenotype. These results suggest that deregulation of miR-455-3p may play a functional role in muscle atrophy and miR-434-3p could be a candidate serum biomarker of muscle aging.
- Subjects
MUSCLE diseases; MORTALITY of older people; DISEASES in older people; MICRORNA; MUSCLE mass; MUSCLE physiology; AGING; ANIMAL experimentation; ANIMALS; BIOCHEMISTRY; BIOLOGICAL models; CELL culture; CELL differentiation; GENES; PHENOMENOLOGY; MICE; MUSCULAR atrophy; POLYMERASE chain reaction; RESEARCH funding; RNA; REVERSE transcriptase polymerase chain reaction; SKELETAL muscle
- Publication
Journals of Gerontology Series A: Biological Sciences & Medical Sciences, 2017, Vol 72, Issue 11, p1483
- ISSN
1079-5006
- Publication type
journal article
- DOI
10.1093/gerona/glx025