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- Title
Nachweis von minimaler Resterkrankung bei malignen B-Zell-Erkrankungen mit Hilfe der Polymerase-Ketten-Reaktion.
- Authors
Moos, Marion; Schulz, Renate; Cremer, F.; Kiel, Katja; Schmohl, Dagmar; Döhner, H.; Goldschmidt, H.; Haas, R.
- Abstract
The prognostic value of the detection of minimal residual disease (MRD) with the help of the polymerase chain reaction (PCR) was investigated in patients with non Hodgkin's lymphoma (NHL), multiple myeloma (MM), and acute myeloid leukemia (AML) of the subclass M4Eo. Basis for the application of a PCR-assay in these disease entities are the following specific gene rearreangements: the t(14;18) translocation in NHL, the clonal rearrangement of the Ig heavy chain locus resulting in a unique complementarity determining region 3 (CDR3) for MM and the Inversion 16 characteristic for the M4Eo subclass of AML. For the (14; 18) marker it could be shown that persistence or re-appearance of t(14;18) positive cells is correlated with a greater relapse probability. In patients with MM, the assessment of MRD in PBSC harvests by PCR showed contamination of the leukapheresis products by tumor cells while experimental preparative flow cytometry resulted in PCR-negativity of all CD34+ fractions. The results of the 13 patients with AML M4Eo and Inversion 16 are preliminary. Examination of the tumor load in autologous progenitor cell grafts from different sources revealed a lower tumor cell contamination in PBSC grafts. In summary, PCR assays may provide important Information on the extent of tumor cell contamination, especially when accompanying new treatment modalities such as enrichment procedures or dose intensification programs.
- Publication
Journal of Laboratory Medicine / Laboratoriums Medizin, 1998, Vol 22, Issue 1, p15
- ISSN
0342-3026
- Publication type
Article