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- Title
指定成分であるコレウス•フォルスコリーを含む健康食品の 定性及び定量分析
- Authors
新井玲子; 政田さやか; 田中誠司; 袴塚高志; 内山奈穂子
- Abstract
In 2020, the government of Japan has designated Coleus forskohlii as "an ingredient calling for special attention" To ensure the efficacy and safety of health food products containing C. forskohlii, it is essential to identify and quantify the active compounds present in that plant. In this study,we established a specific TLC identification method and a quantitative HPLC assay, and evaluated the quality and quantity of 14 health food products. For TLC identification and for a cleaner analysis method, we modified the USP method in which toluene is used as the TLC eluent. Forskolin and its isomer, isoforskolin, were clearly separated with ルhexane/ethyl acetate (1:1) and detected vdth 4-methoxybenzaldehyde/sulfuric acid/ethanol (1:1:18) spray on TLC plates. For HPLC quantification of forskolin in C. forskohlii-containing products, we developed a simpler and more sensitive method based on the USP monograph. Good linearity with a coefficient of determination (r2 > 0.998) was achieved over a concentration range 2-4000)ig/mL. The limit of detection and the limit of quantification were estimated to be 0.42 and 1.28 pg/mL,respectively. The precision of the method was confirmed by 92.01% forskolin recovery. Surprisingly,the forskolin content in health food products varied greatly and ranged up to 300 fold (0.35-120.10 mg/day). Thus,it was considered that the differences in the forskolin content in the sample products were caused by the differences in their merchandise design. These developed methods would be useful for the qualitative and quantitative evaluations of health food products containing C. forskohlii.
- Subjects
FOOD production; HIGH performance liquid chromatography; THIN layer chromatography; ISOMERS; FORSKOLIN
- Publication
Japanese Journal of Food Chemistry & Safety, 2020, Vol 27, Issue 2, p84
- ISSN
1341-2094
- Publication type
Article