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- Title
ラット門脈カテーテル留置法におけるタンパク質消化吸収機能 評価モデルの開発
- Authors
中村衣里; 月向さやか; 冨田勲; 松浦寿喜
- Abstract
We attempted to develop an evaluation model for protein digestion and absorption using the rat portal vein catheter placement method. Sodium caseinate solution was continuously administered from a gastric catheter in rats into which catheters were placed in the stomach and portal vein, and the digestive absorption of protein was measured based on the time course of changes in amino acid concentrations in the portal vein as an index. When 5% sodium caseinate solution was continuously administered (11.25 mL/kg/h) from the gastric catheter using a peristaltic pump, the portal blood concentrations of eight amino acids (glutamine, tyrosine, methionine, tryptophan, valine, phenylalanine, isoleucine and leucine) increased with time. Significant increases were observed for these eight amino acids when compared with pre-administration values (0 minutes) up to 180 minutes after administration. Therefore, the state of protein digestion and absorption can be observed using portal blood amino acid concentrations as indicators. Green tea is reported to suppress protein absorption, and it was therefore investigated whether this inhibitory action could be confirmed using this experimental model. A single administration of a 20% green tea extract (7.2 mL/kg) and continuously administered 5% sodium caseinate solution to rats resulted in a significant inhibitory effect in the portal blood concentrations of these eight amino acids. These results confirm that the rat portal vein catheter placement method is useful as an experimental model for evaluating the digestion and absorption of proteins.
- Publication
Japanese Journal of Food Chemistry & Safety, 2017, Vol 24, Issue 2, p69
- ISSN
1341-2094
- Publication type
Article