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- Title
小檗碱对损伤胰岛βTC6细胞相关信号分子表达的影响.
- Authors
翟佳佳; 王辛; 张艺; 李茉; 路延之; 康健; 邹健; 李泽平; 李晓苗
- Abstract
Objective: The aim of this study was to explore the protective effect of berberine(BBR) on the apoptosis of pancreaticβTC6 cells induced by palmic acid(PA) as well as investigate the role of PTEN and AMPK in BBR involved βTC6 cells protection.Methods: The injury of βTC6 cells was induced by treatment with 1.0 mmol/l PA, and then treated with BBR. The apoptosis of βTC6 cells was evaluated using flow cytometry. The expressions of AMPK and PTEN were estimated utilizing RT-qPCR and immunofluorescence. Results: The proliferation of βTC6 cells was significantly inhibited in PA group compared with that in Con group.Meanwhile, βTC6 cells apoptosis could be induced by PA treatment while BBR attenuated this effect, indicating that BBR played a protective role in the oxidative stress of βTC6 cells. During the occurrence of βTC6 cells apoptosis, the AMPK expression was dramatically reduced while the PTEN expression was significantly increased, suggesting that the expression and transcription of AMPK were inhibited by the severe oxidative damage of βTC6 cells. Besides, PTEN promoted the apoptosis of βTC6 cells. Compared with the PA group, the AMPK expression was increased while the PTEN expression was declined in PA+BBR group, indicating BBR enhanced the anti-oxidative ability of βTC6 cells by activating the AMPK expression and inhibiting the PTEN expression. Conclusions: BBR played a protective effect on PA-induced βTC6 cells apoptosis, and it showed anti-oxidative ability through regulating the expressions of AMPK and PTEN.
- Publication
Progress in Modern Biomedicine, 2019, Vol 19, Issue 10, p1835
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2019.10.007