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- Title
IFITM3 在脓毒症模型及胆碱能抗炎模型中的表达.
- Authors
闫汝虎; 李楠; 张二飞; 涂可; 阴弯弯; 张莉; 杜诗斌; 侯立朝; 孙冬冬
- Abstract
Objective: To investigate the expression of IFITM3 in RAW264.7 cell line stimulated by LPS and Cholinergic anti-inflammatory pathway model. Methods: RAW264.7 cell was treated by 1 μgrnL LPS for 24. 48 and 72 h. the protein expression of IFTTM3 was determined by Western Blot. 50 [Al Cholinergic receptor agonist GTS-21 combined with 100 nM Cholinergic receptor antagonist α-BGT were given after 1 p-g niL LPS stimulation and then the protein expression lewis oflFITND were determined. The model of sepsis and Cholinergic anti-inflammatory pathway were verified by IL-1 μ level detection Results: ① The expression of IHIM3 in RAW264.7 cell lines were greatly decreased after 1 μg/mL LPS stimulation (P<0.01). ② The expression of IFITM3 was significantly increased after treatment with 50 μM GTS-21 for 24 h (P<0.001) but notably declined after treatment with 50 μm GTS for 24 h following 1 μg mL LPS stimulation (P<0.001). Conclusions: The protein expression of ETTM3 was decreased in RAW264.7 cell induced by LPS. Cholinergic receptor agonist GTS-21 could reverse the reduction of IFTTM3 induced by LPS. and this effect was abolished by Cholinergic receptor antagonist μ-BGT. IFTTM3 might have protective effect in sepsis and regulate anti-inflammatory process in Cholinergic antiinflammatory pathway.
- Publication
Progress in Modern Biomedicine, 2018, Vol 18, Issue 12, p2257
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2018.12.012