We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Superovulatory response and embryonic quality of Brahman suckling cows synchronized with low doses of FSH under conditions in southern Mexico.
- Authors
Ponce-Covarrubias, José L.; Mendoza-Medel, Gabriel; Hernández-Ruiz, Pedro E.; Pineda-Burgos, Blanca C.; Valencia-Franco, Edgar; Velázquez-Morales, José V.; García y González, Ethel C.
- Abstract
Objective: Assess the superovulatory response and embryonic quality of a group of suckling Brahman cows undergoing a low dose FSH protocol. Methodology: In the study 33 multiparous suckling Brahman cows were superovulated. For this, the females were synchronized with CIDR (insertion day 0) and decreasing doses of FSH (day 5: 250 mg FSH, day 6: 200 mg FSH, day 7: 150 mg), on day 9 and 10 artificial insemination was performed (IA); finally on day 17 the washing was done to collect the embryos. The embryos were collected with a Foley catheter and the contents were deposited in a 100×l100 mm square Petri dish with a squared bottom for the subsequent search for embryos in the microscope provided with an increase of 20 to 30 X. The embryos were classified according to their morphology and stage of development on a scale from 1 (stage of a cell) to 9 (stage of hatched blast) and quality as 1 (excellent), 2 (good), 3 (regular) and 4 (degenerate). Results: Of the 100% of the cows treated 363 embryos were obtained: 41.8% were viable; 65% were classified as hatched blastocyst of quality 8 and 34.8% of quality 7. Of the embryos classified as hatched blastocyst, 60.6% were of excellent quality, 28.28% of good quality and 11.11% of regular quality. The average of viable embryos per cow was 2.9% and 1.9% transferable. Implications: It's difficult to superovulate lactating cows, due to the control of calves during the experimental process, this type of treatment also is not recommended due to the metabolic and reproductive expenditure to which these females are subjected. Conclusions: Suckling Brahman cows have a low superovulatory response when they are synchronized with a low dose FSH protocol.
- Subjects
MEXICO; COWS; URINARY catheters; EMBRYOS; BLASTOCYST
- Publication
Agro Productividad, 2019, Vol 12, Issue 11, p9
- ISSN
2448-7546
- Publication type
Article
- DOI
10.32854/agrop.vi0.1490