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- Title
Inhibition of KCTD10 Affects Diabetic Retinopathy Progression by Reducing VEGF and Affecting Angiogenesis.
- Authors
Feng, Yun; Wang, Cong; Wang, Guangwei; Mazziotta, Chiara
- Abstract
Aim. We purposed to evaluate the KCTD10 effects of angiogenesis in diabetic retinopathy (DR). Methods. We induced a DR cell model using high glucose (HG) treatment of HRECs and ARPE-19 cells. A DR rat was established by injecting streptozotocin. Small interference RNA targeted KCTD10 (si-KCTD10) was used to mediate KCTD10 inhibition in cell and animal models. The roles of KCTD10 on cell viability, apoptosis, angiogenesis, and related proteins (VEGF and HIF-1 α) were observed by RT-qPCR, Western blot, CCK-8 assay, TUNEL staining, tube formation assay, ELISA, and immunohistochemistry assay. Results. KCTD10 expression was upregulated in DR cells and retinal tissue of DR rats. Treatment of the cells with si-KCTD10 increased cell viability and decreased apoptosis and angiogenesis in DR cells. Inhibition of KCTD10 could reduce the expression of VEGF and HIF-1 α in DR cells. Furthermore, KCTD10 inhibition reduced VEGF levels in the retinal tissue of DR rats. Conclusion. This work showed that inhibition of KCTD10 relieved angiogenesis in DR.
- Subjects
DIABETIC retinopathy; NEOVASCULARIZATION; NON-coding RNA; CELL survival; STREPTOZOTOCIN; WESTERN immunoblotting
- Publication
Genetics Research, 2022, Vol 2022, p1
- ISSN
0016-6723
- Publication type
Article
- DOI
10.1155/2022/4112307