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- Title
Assessment of the Virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice.
- Authors
Gaspar, Emanuelle Baldo; Santos, Lenita Ramires dos; Egito, Andréa Alves do; Santos, Maria Goretti dos; Mantovani, Cynthia; Rieger, Juliana da Silva Gomes; Abrantes, Guilherme Augusto de Sousa; Suniga, Paula Adas Pereira; Favacho, Júlia de Mendonça; Pinto, Ingrid Batista; Nassar, Alessandra Figueiredo de Castro; Santos, Fernando Leandro dos; Araújo, Flábio Ribeiro de
- Abstract
Burkholderia mallei is an aerobic, Gram-negative, non-motile bacillus. As an obligate mammalian pathogen, it primarily affects solipeds. Although rarely transmitted to humans, the disease it causes, glanders, is classified as a zoonosis. The bacterium was officially eradicated in Brazil in 1969; however, it reemerged after three decades. This study aims to assess the virulence of a specific B. mallei strain, isolated in Brazil, in BALB/c mice through intranasal infection. The strain, B. mallei BAC 86/19, was obtained from the tracheal secretion of a young mare displaying positive serology but no clinical signs of glanders. Post-mortem examinations revealed macroscopic lesions consistent with the disease, however. In mice, the LD50 was determined to be approximately 1.59 × 105 colony-forming units (CFU)/animal. Mice exposed to either 0.1 × LD50 or 1 × LD50 displayed transient weight loss, which resolved after three or five days, respectively. B. mallei persisted within the liver and lung for five days post-infection and in the spleen for seven days. These findings underscore the detectable virulence of the Brazilian B. mallei BAC 86/19 strain in mice, which are relatively resilient hosts. This research points to the importance of the continued investigation of the virulence mechanisms and potential countermeasures associated with B. mallei infections, including their Brazilian isolates.
- Subjects
BRAZIL; BURKHOLDERIA; AUTOPSY; GRAM-negative bacteria; MICE; WEIGHT loss; SYMPTOMS
- Publication
Microorganisms, 2023, Vol 11, Issue 10, p2597
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms11102597