We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Direct Identification of On-Bead Peptides Using Surface-Enhanced Raman Spectroscopic Barcoding System for High-Throughput Bioanalysis.
- Authors
Kang, Homan; Hong Jeong, Dae; Jeong, Sinyoung; Geun Cha, Myeong; Chang, Hye-Jin; Koh, Yul; Kim, Yong-Kweon; Yang, Jin-Kyoung; Kyeong, San; Kim, Jaehi; Kwak, Seon-Yeong; Lee, Hyunmi; Jeong, Cheolhwan; Kim, Jong-Ho; Jun, Bong-Hyun; Lee, Yoon-Sik
- Abstract
Recently, preparation and screening of compound libraries remain one of the most challenging tasks in drug discovery, biomarker detection, and biomolecular profiling processes. So far, several distinct encoding/decoding methods such as chemical encoding, graphical encoding, and optical encoding have been reported to identify those libraries. In this paper, a simple and efficient surface-enhanced Raman spectroscopic (SERS) barcoding method using highly sensitive SERS nanoparticles (SERS ID) is presented. The 44 kinds of SERS IDs were able to generate simple codes and could possibly generate more than one million kinds of codes by incorporating combinations of different SERS IDs. The barcoding method exhibited high stability and reliability under bioassay conditions. The SERS ID encoding based screening platform can identify the peptide ligand on the bead and also quantify its binding affinity for specific protein. We believe that our SERS barcoding technology is a promising method in the screening of one-bead-one-compound (OBOC) libraries for drug discovery.
- Subjects
SERS spectroscopy; PEPTIDE synthesis; BIOMARKERS; BIOLOGICAL assay; OLIGONUCLEOTIDES
- Publication
Scientific Reports, 2015, p10144
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep10144