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- Title
Molecular Detection of Leptospira spp. Isolated from Aborted Ovine Genital Swabs in Lorestan, Iran, 2019-2020.
- Authors
Anari, Shabnam; Jaydari, Amin; Shams, Nemat; Rahimi, Heidar
- Abstract
Background and Aim: Leptospirosis is a zoonotic bacterial disease caused by the genus Leptospira. This disease is observed in humans, domestic and wild mammals. Abortion is known as the most important side effect of Leptospira in infected sheep. Molecular tests such as PCR are considered for the early detection of disease which cannot trigger the production of speciesspecific antibodies. This study was designed to detect Leptospira spp. as a cause of abortion in sheep using PCR in Lorestan province, Iran. Materials and Methods: In the current study a total of 150 samples of vaginal swabs were randomly collected from a sheep that had an abortion in some areas of Lorestan province, Iran. The collected samples were accurately investigated to trace the DNA of Leptosoira spp. using specific primers. Results & Conclusion: Based on results of Leptosoira spp. lipL32 gene, 2 positive samples (1.3%) were identified. Hence, it was concluded that there is no significant relationship between the abortion rate and Leptospirosis in Lorestan province, Iran. It must be mentioned that due to the zoonotic nature of this bacterium, rapid diagnosis of this disease in livestock can extremely decrease the outbreak of this disease in humans.
- Subjects
IRAN; DNA analysis; RISK factors in miscarriages; LEPTOSPIROSIS diagnosis; SHEEP; GRAM-negative bacteria; ANIMAL experimentation; RISK assessment; EPIDEMICS; GENES; RESEARCH funding; POLYMERASE chain reaction
- Publication
Iranian Journal of Medical Microbiology, 2023, Vol 17, Issue 1, p107
- ISSN
1735-8612
- Publication type
Article
- DOI
10.30699/ijmm.17.1.107