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- Title
SGOL1 variant B induces abnormal mitosis and resistance to taxane in non-small cell lung cancers.
- Authors
Shun Matsuura; Tomoaki Kahyo; Kazuya Shinmura; Moriya Iwaizumi; Hidetaka Yamada; Kazuhito Funai; Jun Kobayashi; Masayuki Tanahashi; Hiroshi Niwa; Hiroshi Ogawa; Takashi Takahashi; Naoki Inui; Takafumi Suda; Kingo Chida; Yoshinori Watanabe; Haruhiko Sugimura
- Abstract
Mitosis is the most conspicuous cell cycle phase and Shugoshin-like 1 (SGOL1) is a key protein in protecting sister chromatids from precocious separation during mitosis. We studied the role of SGOL1 and its splice variants in non-small cell lung cancer (NSCLC) using 82 frozen NSCLC tissue samples. SGOL1-B expression was prevalent in smokers, in cases with a wild-type (WT) EGFR status, and in cases with the focal copy number amplification of genes that are known to be important for defining the biological behaviors of NSCLC. The overexpression of SGOL1-B1 in an NSCLC cell line induced aberrant chromosome missegregation, precociously separated chromatids, and delayed mitotic progression. A higher level of SGOL1-B mRNA was related to taxane resistance, while the forced downregulation of SGOL1-B increased the sensitivity to taxane. These results suggest that the expression of SGOL1-B causes abnormal mitosis and taxane resistance in NSCLC cells.
- Subjects
MITOSIS; TAXANES; CIGARETTE smokers; SMALL cell lung cancer; HUMAN chromosome abnormalities; GENE amplification
- Publication
Scientific Reports, 2013, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep03012