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- Title
Unravelling the Gastroprotective Potential of Kefir: Exploring Antioxidant Effects in Preventing Gastric Ulcers.
- Authors
Côco, Larissa Zambom; Aires, Rafaela; Carvalho, Glaucimeire Rocha; Belisário, Eduarda de Souza; Yap, Michelle Khai Khun; Amorim, Fernanda Gobbi; Conde-Aranda, Javier; Nogueira, Breno Valentim; Vasquez, Elisardo Corral; Pereira, Thiago de Melo Costa; Campagnaro, Bianca Prandi
- Abstract
The present study was conducted to evaluate the protective effect of milk kefir against NSAID-induced gastric ulcers. Male Swiss mice were divided into three groups: control (Vehicle; UHT milk at a dose of 0.3 mL/100 g), proton pump inhibitor (PPI; lansoprazole 30 mg/kg), and 4% milk kefir (Kefir; 0.3 mL/100 g). After 14 days of treatment, gastric ulcer was induced by oral administration of indomethacin (40 mg/kg). Reactive oxygen species (ROS), nitric oxide (NO), DNA content, cellular apoptosis, IL-10 and TNF-α levels, and myeloperoxidase (MPO) enzyme activity were determined. The interaction networks between NADPH oxidase 2 and kefir peptides 1–35 were determined using the Residue Interaction Network Generator (RING) webserver. Pretreatment with kefir for 14 days prevented gastric lesions. In addition, kefir administration reduced ROS production, DNA fragmentation, apoptosis, and TNF-α systemic levels. Simultaneously, kefir increased NO bioavailability in gastric cells and IL-10 systemic levels. A total of 35 kefir peptides showed affinity with NADPH oxidase 2. These findings suggest that the gastroprotective effect of kefir is due to its antioxidant and anti-inflammatory properties. Kefir could be a promising natural therapy for gastric ulcers, opening new perspectives for future research.
- Subjects
ANTIOXIDANTS; STOMACH ulcers; KEFIR; ORAL drug administration; NADPH oxidase; REACTIVE oxygen species
- Publication
Cells (2073-4409), 2023, Vol 12, Issue 24, p2799
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells12242799