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- Title
Allergen components of Dermatophagoides farinae recognised by serum immunoglobulin (Ig)E in Thai dogs with atopic dermatitis.
- Authors
Khantavee, Nathrada; Reamtong, Onrapak; Sookrung, Nitat; Suradhat, Sanipa; Prapasarakul, Nuvee
- Abstract
Background: Dermatophagoides farinae (Der f) is a common allergen in dogs with atopic dermatitis (AD). However, the relevant components of Der f require further investigation. Objectives: We aimed to provide data on the immunoglobulin (Ig)E‐binding specific components of Der f for further diagnostic and therapeutic applications. Animals: Serum samples were collected from five healthy, nine Der f‐allergic atopic and seven non‐Der f‐allergic atopic dogs identified based on an intradermal skin test. Methods and materials: We explored the component profiles of Der f extracts through 2D gel electrophoresis and IgE immunoblotting. The IgE‐binding components in both groups of atopic dogs were analysed by mass spectrometry. Results: The majority of Der f‐allergic atopic dogs recognised Der f Alternaria alternata allergen 10 (Der f Alt a 10), elongation factor 1‐alpha (EF1‐α), gelsolin‐like allergen Der f 16, Der f 28 and Der f 2. Der f 3, Der f 10, Der f 20 and Der f 32 were recognised as minor allergens. Alpha‐enolase, serine protease, arginine kinase and a few hypothetical proteins were recognised components in both groups of atopic dogs. Unexpectedly, Der f 15 (chitinase) was found to be a minor component. Conclusions and clinical importance: Multiple IgE‐binding allergens of Der f were identified in Thai atopic dogs. We propose that the specific antigen set that is bound by IgE, comprising Der f Alt a 10, EF1‐α, gelsolin‐like Der f 16, Der f 28 and Der f 2, could be used for future diagnostics and immunotherapy platforms.
- Subjects
ATOPIC dermatitis; ALLERGENS; DERMATOPHAGOIDES; IMMUNOGLOBULIN E; DOGS; ALTERNARIA alternata; FILAGGRIN
- Publication
Veterinary Dermatology, 2021, Vol 32, Issue 4, p338
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/vde.12967