We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Site-Specific Transformation of Drosophila via ɸC31 Integrase-Mediated Cassette Exchange.
- Authors
Bateman, Jack R.; Lee, Anne M.; C.-ting Wu
- Abstract
Position effects can complicate transgene analyses. This is especially true when comparing transgenes that have inserted randomly into different genomic positions and are therefore subject to varying position effects. Here, we introduce a method for the precise targeting of transgenic constructs to predetermined genomic sites in Drosophila using the ΦC31 integrase system in conjunction with recombinase-mediated cassette exchange (RMCE). We demonstrate the feasibility of this system using two donor cassettes, one carrying the yellow gene and the other carrying GFP At all four genomic sites tested, we observed exchange of donor cassettes with an integrated target cassette carrying the mini-while gene. Furthermore, because RMCE-mediated integration of the donor cassette is necessarily accompanied by loss of the target cassette, we were able to identify integrants simply by the loss of mini-white eye color. Importantly, this feature of the technology will permit integration of unmarked constructs into Drosophila, even those lacking functional genes. Thus, ΦC31 integrase-mediated RMCE should greatly facilitate transgene analysis as well as permit new experimental designs.
- Subjects
TRANSGENES; DROSOPHILA; GENOMICS; GENETICS; GENES; DROSOPHILIDAE
- Publication
Genetics, 2006, Vol 173, Issue 2, p769
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.106.056945