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- Title
Division of Labor Among the Yeast Sol Proteins Implicated in tRNA Nuclear Export and Carbohydrate Metabolism.
- Authors
Stanford, D. R.; Whitney, M. L.; Hurto, R. L.; Eisaman, D. M.; Shen, W.-C.; Hopper, A. K.
- Abstract
SOL1, the founding member of the S. cerevisiae SOL family, was previously identified as a multi-copy suppressor of the los1 defect in tRNA-mediated nonsense suppression. Here we report that the four-member SOL family is not essential and that individual family members appear to have distinct functions. SOL1-SOL4 are homologous to genes encoding 6-phosphogluconolactonase (6Pgl) involved in the pentose phosphate pathway. Both So13p and So14p affect this activity. However, So14p does not act as a los1 multi-copy suppressor. In contrast, neither So11p nor So12p, both of which correct the los1 defect in nonsense suppression, possess detectable 6Pg1 activity. Rather, So11p and So12p appear to function in tRNA nuclear export as sol1 and so12 mutants possess elevated levels of nuclear tRNA. Members of the Sol protein family appear to have different subcellular distributions. Thus, So13p and So14p likely function in carbohydrate metabolism, while So11p and So12p appear to have roles in tRNA function and nuclear export, thereby defining an unusual protein family whose individual members are biochemically distinct and spatially dispersed.
- Subjects
NONSENSE suppression (Genetics); GENETICS; PROTEINS; CARBOHYDRATE metabolism; BIOCHEMISTRY; SACCHAROMYCES cerevisiae
- Publication
Genetics, 2004, Vol 168, Issue 1, p117
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.104.030452