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- Title
Latex Agglutination as an Alternative to the Hemagglutination Reaction of Influenza Viruses.
- Authors
Ivanov, P. A.; Lyashko, A. V.; Ionov, S. A.; Shcherbinin, D. N.; Rudneva, I. A.; Shilov, A. A.; Bunkova, N. I.; Shmarov, M. M.; Timofeeva, T. A.
- Abstract
Abstract—As an alternative to the classical method of erythrocyte hemagglutination, a latex agglutination assay based on the interaction of influenza viruses with the sialoglycoprotein fetuin immobilized on the surface of polystyrene microspheres has been developed. Twelve influenza A virus strains of different subtypes and two influenza B viruses of different lines were tested. Simultaneous titration of viruses using the classical hemagglutination test and the proposed latex agglutination assay showed similar sensitivity and a high degree of correlation (R = 0.94). The obtained microspheres can be used for titration of viruses that recognize and bind sialylated glycans as receptors. In particular, latex aggregation was also induced by the Newcastle disease virus.
- Subjects
INFLUENZA viruses; NEWCASTLE disease virus; LATEX; AGGLUTINATION; BLOOD agglutination; INFLUENZA A virus
- Publication
Molecular Biology, 2023, Vol 57, Issue 5, p913
- ISSN
0026-8933
- Publication type
Article
- DOI
10.1134/S0026893323040088