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- Title
Cell cycle regulated expression and phosphorylation of hpttg proto-oncogene product.
- Authors
Ramos-Morales, Francisco; Domínguez, África; Romero, Francisco; Luna, Rosa; Multon, Marie-Christine; Pintor-Toro, José A; Tortolero, María
- Abstract
We recently isolated a cDNA for hpttg, the human homolog of rat pituitary tumor transforming gene. Now we have analysed the expression of hpttg as a function of cell proliferation. hPTTG protein level is up-regulated in rapidly proliferating cells, is down-regulated in response to serum starvation or cell confluence, and is regulated in a cell cycle-dependent manner, peaking in mitosis. In addition, we show that hPTTG is phosphorylated during mitosis. Immunodepletion and in vitro phosphorylation experiments, together with the use of a specific inhibitor, indicate that Cdc2 is the kinase that phosphorylates hPTTG. These results suggest that hpttg is induced by, and may have a role in, regulatory pathways involved in the control of cell proliferation. Oncogene (2000) 19, 403–409.
- Subjects
CELL cycle; GENE expression; PHOSPHORYLATION; PROTO-oncogenes; TUMORS
- Publication
Oncogene, 2000, Vol 19, Issue 3, p403
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1203320