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- Title
Continuous Packed Bed Reactor with Immobilized β-Galactosidase for Production of Galactooligosaccharides (GOS).
- Authors
Rodriguez-Colinas, Barbara; Fernandez-Arrojo, Lucia; Santos-Moriano, Paloma; Ballesteros, Antonio O.; Plou, Francisco J.
- Abstract
The β-galactosidase from Bacillus circulans was covalently attached to aldehyde-activated (glyoxal) agarose beads and assayed for the continuous production of galactooligosaccharides (GOS) in a packed-bed reactor (PBR). The immobilization was fast (1 h) and the activity of the resulting biocatalyst was 97.4 U/g measured with o-nitrophenyl-β-D-galactopyranoside (ONPG). The biocatalyst showed excellent operational stability in 14 successive 20 min reaction cycles at 45˚Cin a batch reactor. A continuous process for GOS synthesis was operated for 213 h at 0.2 mL/min and45˚C using 100 g/L of lactose as a feed solution. The efficiency of the PBR slightly decreased withtime; however, the maximum GOS concentration (24.2 g/L) was obtained after 48 h of operation, which corresponded to 48.6% lactose conversion and thus to maximum transgalactosylation activity. HPAEC-PAD analysis showed that the two major GOS were the trisaccharideGal-β (1!4)-Gal-β (1!4)-Glc and the tetrasaccharide Gal-β(1!4)-Gal-β(1!4)-Gal-β(1!4)-Glc.The PBR was also assessed in the production of GOS from milk as a feed solution. The stability of the bioreactor was satisfactory during the first 8 h of operation; after that, a decrease in the flow rate was observed, probably due to partial clogging of the column. This work represents a step forward in the continuous production of GOS employing fixed-bed reactors with immobilized β-galactosidases.
- Subjects
PACKED bed reactors; BACILLUS circulans; GALACTOSIDASES
- Publication
Catalysts (2073-4344), 2016, Vol 6, Issue 12, p189
- ISSN
2073-4344
- Publication type
Article
- DOI
10.3390/catal6120189